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Build Your Reliable Predictive System
with Highly Functional SynFire® iNs

NeuCyte provides SynFire iNs, which are pure, ready-to-use induced human pluripotent stem cell (iPSC)-derived neurons. The different neuronal subtypes include Glutamatergic excitatory iNs, GABAergic inhibitory iNs and astroglia. Such neuronal subtype identities have been well characterized by a comprehensive set of functional assays.

SynFire Neurons and Astroglia can be purchased individually or as kits for co-culture and MEA studies. Various pack sizes and kit configurations are available. We can also customize kit configurations for you.

For detailed ordering information, please review our User Guide

  • Real human biology: These cells closely resemble real human biology, resulting in better ability to predict responses to compounds.
    NeuCyte’s iNs demonstrate principal neurophysiological properties Patch-clamp studies show mature intrinsic and extrinsic properties in SynFire® neural cultures including (A) voltage-dependent K+ and Na+currents, (B) action potential firing (evoked). SynFire iNs show, (C) bursting of single neurons, and (D) large postsynaptic currents indicating advance synaptic competence. Pure SynFire subtype cultures (E) of either (i) only excitatory iNs or (ii) only inhibitory iNs exclusively show glutamate mediated excitatory postsynaptic currents (EPSCs) or GABA-mediated inhibitory postsynaptic currents (IPSCs), respectively. SynFire iNs are suited for studying short and long term plasticity and show (E) robust NMDA currents starting at five weeks in culture. Moreover, extra-synaptic NMDA currents can be specifically analyzed by (F) co-application of activating glutamate and glycine in the presence of the NMDA inhibitor D-AP5. SynFire neural cultures rapidly mature within five weeks (G) reaching a resting membrane potential <-60 mV and showing stable excitability (action potential threshold and overshoot).
  • Rapid maturation: Produced through a direct reprogramming approach leading to rapid and homogeneous maturation, SynFire iNs exhibit mature synaptic network activity, such as synchronous bursting phenotypes resembling those in rodent primary cultures appearing within three to four weeks.
    Ontogeny of neural network activity maturation of SynFire co-cultures These co-cultures contain 70% Glutamatergic, 30% GABAergic neurons and human astrocytes. Representative raster plots from MEA recordings at weeks 1-4. Axion 48 well MEA plates were used to assess activity.
  • Reliable, robust and ready-to-use: This reprogramming approach also results in lot-to-lot consistency, providing reproducible results.
    SynFire iNs show stable baseline neuronal activity Neuronal firing and bursting characteristics demonstrated by SynFire iNs show little variability across batches and individuals. These cells also exhibit low number of outlier wells of technical replicates.
  • Flexible modular system: The user can control subtype to subtype relative seeding density and ratio, in order to track, analyze and manipulate specific cell types to fit individual projects.
    Neurite outgrowth assay using SynFire® iN co-cultures treated with actin filament disruptive toxin SynFire® neural cultures were treated with the actin filament disruptive toxin Latrunculin A (100 nM). Neurite length was assessed and quantified over a period of 44 hours using a live imaging Incucyte system. Representative images of the neurite traces from both excitatory and inhibitory neurons are included.

Advantages of SynFire iNs include:

SynFire iN cells represent a versatile in vitro cell system for research and disease modeling. These cells can also be used for compound screening as well as nonclinical safety assessment and chemical neurotoxicity studies.

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